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Copyright (c) 2017 Irides Chaverra Mendoza
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Usefulness of immunological and molecular methods for the diagnosis of Chikungunya virus
Corresponding Author(s) : Irides Chaverra Mendoza
Ciencia e Innovación en Salud,
2017
Abstract
Background: Chikungunya is a virus that has caused many infectious outbreaks worldwide becoming a public health problem. It shares several similarities with the Dengue virus, are transmitted by the same vector that is a mosquito of the genus Aedes, are found in tropical and subtropical areas and present the same clinical symptoms in the acute phase of the disease, which makes its clinical diagnosis difficult. Methods: Bibliographic revision of the diagnostic methods of the virus from the world literature in searchers of database and indexed journals. Discussion: Among the results it can be said that of all the immunological techniques, the MAC ELISA formats for antibody detection and the ELISAs for antigen detection show reliable results for the identification of the virus, with respect to the molecular tests it can be affirmed that they are highly sensitive and specific techniques, with multiple advantages over immunological techniques since they can detect and quantify both viruses simultaneously. Conclusion: The best techniques are molecular tests, but it is very expensive for most laboratories, therefore, the use of ELISA assays based on antigen detection is recommended because they are specific, sensitive and can detect the virus in the acute phase of the disease.
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- Lo Presti A, Lai A, Cella E, Zehender G, Ciccozzi M. Chikungunya virus, epidemiology, clinics and phylogenesis: A review. Asian Pacific Journal of Tropical Medicine. 2014; 7(12): 925-932.
- Loreto Horcada M, Diaz caldero C, Garrido L. Fiebre chikungunya. Manifestaciones reumáticas de una infección emergente en Europa. Reumatología Clinica.2014; 11(3): 161-164.
- Razmy A. Clinical features of chikungunya infection in Sri Lanka. Asian Pacific Journal of Tropical Disease.2014;4(2):131-134.
- Plan nacional de respuesta frente a la introducción Del virus chikungunya en colombia. Plan de Respuesta a la Fiebre por el Virus Chikungunya. Agosto de 2014: 1-20. http://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ET/PLAN%20NACIONAL%20DE%20RESPUESTA%20CHIKUNGUNYA%20COLOMBIA%202014.pdf. (Ultimo acceso 30 de Marzo 2015).
- Nkoghe D, Kassa R, Bisvigou U, Caron M, Grard G. No clinical or biological difference between Chikungunya and Dengue Fever during the 2010 Gabonese outbreak. Infectious Disease Report. 2012; 4(5):11-13.
- Javier de la Torre Galvis. Habrá Chikungunya para rato. Revista Semana. 3 de Enero de 2015.Edicion http://www.semana.com/nacion/articulo/chikungunya-estara-en-colombia-largo-tiempo/413768-3 ( ultimo acceso 30 de marzo 2015)
- Vigilancia rutinaria por eventos municipal 2014. Instituto Nacional de Salud, Portal Sivigila, Sistema Nacional de Vigilancia en Salud Publica.2014. http://www.ins.gov.co/lineas-deaccion/SubdireccionVigilancia/sivigila/Paginas/vigilancia-rutinaria.aspx (ultimo acceso 30 de Marzo 2015).
- Vigilancia rutinaria por eventos municipal 2015. Instituto Nacional de Salud, Portal Sivigila, Sistema Nacional de Vigilancia en Salud Publica.2015. http://www.ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/sivigila/Paginas/vigilancia-rutinaria.aspx (ultimo acceso 30 de Marzo 2015).
- Tattevin P. Dengue, chikungunya : deux arboviroses emergentes. Journal des Anti-infectieux.2012; 14:89-95.
- Pongsiri P, Praianantathavorn K, Theamboonlers A, Payungporn S, Poovorawan Y. Multiplex real –time RT-PCR for detecting Chikungunya virus and Dengue virus. Asian Pacific Journal of Tropical Medicine. 2012; 5(5): 342-346.
- Bedoya Arias J, Murillo Garcia D, Bolaños Muñoz E, Hurtado Hurtado N, Ramirez Jaramillo V, et al. Healthcare students and workers’ knowledge about epidemiology and symptoms of chikungunya fever in two cities of Colombia. The Journal Of Infection In Developing Country.2015; 9(3): 330-332.
- Lineamientos de vigilancia en salud pública, entomológica y de laboratorio ante la introducción del virus chikungunya. Anexo 3 Lineamientos de vigilancia CHKV 2014. Instituto nacional De salud. Julio 2014:1-15. http://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ET/Anexo%203%20Lineamientos%20de%20Vigilancia%20CHIKV%202014.pdf. (Ultimo acceso 30 de Marzo 2015).
- Semana epidemiológica número 52 de 2015. Boletín Epidemiológico semanal. 27 dic. Al 02 enero 2016: 26-30. http://www.ins.gov.co/boletin-epidemiologico/Boletn%20Epidemiolgico/2015%20Boletin%20epidemiologico%20semana%2015.pdf (ultimo acceso 24 Mayo de 2016).
- Actualización de lineamientos de la notificación de casos de virus Chikungunya en Colombia Fase II. Circular externa 0001 del 8 de enero de 2015. http://www.ins.gov.co/Noticias/Chikungunya/Circular%20001-2015.pdf ( ultimo acceso 27 de abril 2015)
- Reddy V, Ravi V, Desai A, Parida M , Powers A, et al. Utility of IgM ELISA, TaqMan Real-Time PCR, Reverse Transcription PCR, and RT-LAMP Assay for the Diagnosis of Chikungunya Fever. Journal of Medical Virology. 2012; 84: 1771-1778.
- Kashyap R, Morey S, Chandak N, Purohit H, Taori G, et al. Detection of viral antigen, IgM and IgG antibodies in cerebrospinal fluid of Chikungunya patients with neurological complications. Cerebrospinal Fluid Research. 2010;
- Yathi K, Bhasker S, Chinnamma M. Determination of B cell epitopes and evaluation of antigen capture ELISA for the earlier diagnosis of CHIK virus using anti-rCHIK E1 rabbit antibodies. Journal of Inmunological Methods. 2013; 393: 45-52.
- 7(12). http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927496/pdf/1743-8454-7-12.pdf (ultimo acceso 13 Mayo 2015).
- Prat C, Flusin O, Panella A, Tenebray B, Lanciotti R, et al. Evaluation of Commercially Available Serologic Diagnostic Tests for Chikungunya Virus. Emerging Infectious Diseases. 2014; 20(12): 2129-2132.
- Okabayashi T, Sasaki T, Masrinoul P, Chantawat N, Yoksan S, et al. Detection of Chikungunya Virus Antigen by a Novel Rapid Immunochromatographic. Journal of Clinical Microbiology. 2015; 53(2): 382-388.
- Saha K, Firdaus R, Chakrabarti S, Chandra Sadhukhan P. Development of rapid, sensitive one-tube duplex RT-PCR assay for specific and differential diagnosis of Chikungunya and dengue. Journal of Virology Methods. 2013; 193:521-524.
- Mishra B, Sharma M, Pujhari S, Ratho R, Gopal D, et al. Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and Chikungunya viruses in clinical samples. Diagnostic Microbiology and Infectious Disease. 2011; 71:118–125.
- Cecilia D, Kakade M, Alagarasu K, Patil J,Salunke A, et al. Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses. Archives Virology. 2015; 160:323-327.
References
Lo Presti A, Lai A, Cella E, Zehender G, Ciccozzi M. Chikungunya virus, epidemiology, clinics and phylogenesis: A review. Asian Pacific Journal of Tropical Medicine. 2014; 7(12): 925-932.
Loreto Horcada M, Diaz caldero C, Garrido L. Fiebre chikungunya. Manifestaciones reumáticas de una infección emergente en Europa. Reumatología Clinica.2014; 11(3): 161-164.
Razmy A. Clinical features of chikungunya infection in Sri Lanka. Asian Pacific Journal of Tropical Disease.2014;4(2):131-134.
Plan nacional de respuesta frente a la introducción Del virus chikungunya en colombia. Plan de Respuesta a la Fiebre por el Virus Chikungunya. Agosto de 2014: 1-20. http://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ET/PLAN%20NACIONAL%20DE%20RESPUESTA%20CHIKUNGUNYA%20COLOMBIA%202014.pdf. (Ultimo acceso 30 de Marzo 2015).
Nkoghe D, Kassa R, Bisvigou U, Caron M, Grard G. No clinical or biological difference between Chikungunya and Dengue Fever during the 2010 Gabonese outbreak. Infectious Disease Report. 2012; 4(5):11-13.
Javier de la Torre Galvis. Habrá Chikungunya para rato. Revista Semana. 3 de Enero de 2015.Edicion http://www.semana.com/nacion/articulo/chikungunya-estara-en-colombia-largo-tiempo/413768-3 ( ultimo acceso 30 de marzo 2015)
Vigilancia rutinaria por eventos municipal 2014. Instituto Nacional de Salud, Portal Sivigila, Sistema Nacional de Vigilancia en Salud Publica.2014. http://www.ins.gov.co/lineas-deaccion/SubdireccionVigilancia/sivigila/Paginas/vigilancia-rutinaria.aspx (ultimo acceso 30 de Marzo 2015).
Vigilancia rutinaria por eventos municipal 2015. Instituto Nacional de Salud, Portal Sivigila, Sistema Nacional de Vigilancia en Salud Publica.2015. http://www.ins.gov.co/lineas-de-accion/Subdireccion-Vigilancia/sivigila/Paginas/vigilancia-rutinaria.aspx (ultimo acceso 30 de Marzo 2015).
Tattevin P. Dengue, chikungunya : deux arboviroses emergentes. Journal des Anti-infectieux.2012; 14:89-95.
Pongsiri P, Praianantathavorn K, Theamboonlers A, Payungporn S, Poovorawan Y. Multiplex real –time RT-PCR for detecting Chikungunya virus and Dengue virus. Asian Pacific Journal of Tropical Medicine. 2012; 5(5): 342-346.
Bedoya Arias J, Murillo Garcia D, Bolaños Muñoz E, Hurtado Hurtado N, Ramirez Jaramillo V, et al. Healthcare students and workers’ knowledge about epidemiology and symptoms of chikungunya fever in two cities of Colombia. The Journal Of Infection In Developing Country.2015; 9(3): 330-332.
Lineamientos de vigilancia en salud pública, entomológica y de laboratorio ante la introducción del virus chikungunya. Anexo 3 Lineamientos de vigilancia CHKV 2014. Instituto nacional De salud. Julio 2014:1-15. http://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ET/Anexo%203%20Lineamientos%20de%20Vigilancia%20CHIKV%202014.pdf. (Ultimo acceso 30 de Marzo 2015).
Semana epidemiológica número 52 de 2015. Boletín Epidemiológico semanal. 27 dic. Al 02 enero 2016: 26-30. http://www.ins.gov.co/boletin-epidemiologico/Boletn%20Epidemiolgico/2015%20Boletin%20epidemiologico%20semana%2015.pdf (ultimo acceso 24 Mayo de 2016).
Actualización de lineamientos de la notificación de casos de virus Chikungunya en Colombia Fase II. Circular externa 0001 del 8 de enero de 2015. http://www.ins.gov.co/Noticias/Chikungunya/Circular%20001-2015.pdf ( ultimo acceso 27 de abril 2015)
Reddy V, Ravi V, Desai A, Parida M , Powers A, et al. Utility of IgM ELISA, TaqMan Real-Time PCR, Reverse Transcription PCR, and RT-LAMP Assay for the Diagnosis of Chikungunya Fever. Journal of Medical Virology. 2012; 84: 1771-1778.
Kashyap R, Morey S, Chandak N, Purohit H, Taori G, et al. Detection of viral antigen, IgM and IgG antibodies in cerebrospinal fluid of Chikungunya patients with neurological complications. Cerebrospinal Fluid Research. 2010;
Yathi K, Bhasker S, Chinnamma M. Determination of B cell epitopes and evaluation of antigen capture ELISA for the earlier diagnosis of CHIK virus using anti-rCHIK E1 rabbit antibodies. Journal of Inmunological Methods. 2013; 393: 45-52.
7(12). http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927496/pdf/1743-8454-7-12.pdf (ultimo acceso 13 Mayo 2015).
Prat C, Flusin O, Panella A, Tenebray B, Lanciotti R, et al. Evaluation of Commercially Available Serologic Diagnostic Tests for Chikungunya Virus. Emerging Infectious Diseases. 2014; 20(12): 2129-2132.
Okabayashi T, Sasaki T, Masrinoul P, Chantawat N, Yoksan S, et al. Detection of Chikungunya Virus Antigen by a Novel Rapid Immunochromatographic. Journal of Clinical Microbiology. 2015; 53(2): 382-388.
Saha K, Firdaus R, Chakrabarti S, Chandra Sadhukhan P. Development of rapid, sensitive one-tube duplex RT-PCR assay for specific and differential diagnosis of Chikungunya and dengue. Journal of Virology Methods. 2013; 193:521-524.
Mishra B, Sharma M, Pujhari S, Ratho R, Gopal D, et al. Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and Chikungunya viruses in clinical samples. Diagnostic Microbiology and Infectious Disease. 2011; 71:118–125.
Cecilia D, Kakade M, Alagarasu K, Patil J,Salunke A, et al. Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses. Archives Virology. 2015; 160:323-327.