Usefulness of immunological and molecular methods for the diagnosis of Chikungunya virus

Main Article Content

Irides Chaverra Mendoza


Chikungunya diagnóstico, RT-PCR, MAC-ELISA


Background: Chikungunya is a virus that has caused many infectious outbreaks worldwide becoming a public health problem. It shares several similarities with the Dengue virus, are transmitted by the same vector that is a mosquito of the genus Aedes, are found in tropical and subtropical areas and present the same clinical symptoms in the acute phase of the disease, which makes its clinical diagnosis difficult. Methods: Bibliographic revision of the diagnostic methods of the virus from the world literature in searchers of database and indexed journals. Discussion: Among the results it can be said that of all the immunological techniques, the MAC ELISA formats for antibody detection and the ELISAs for antigen detection show reliable results for the identification of the virus, with respect to the molecular tests it can be affirmed that they are highly sensitive and specific techniques, with multiple advantages over immunological techniques since they can detect and quantify both viruses simultaneously. Conclusion: The best techniques are molecular tests, but it is very expensive for most laboratories, therefore, the use of ELISA assays based on antigen detection is recommended because they are specific, sensitive and can detect the virus in the acute phase of the disease.


Download data is not yet available.
Abstract 737 | PDF Downloads 0 HTML Downloads 0 XML Downloads 0


Bedoya Arias J, Murillo Garcia D, Bolaños Muñoz E, Hurtado Hurtado N, Ramirez Jaramillo V, et al. 20 Healthcare students and workers’ knowledge about epidemiology and symptoms of chikungunya fever in two cities of Colombia. J Infect Dev Ctries 9(3): 330-332. DOI 10.3855/jidc.6445

Cecilia D, Kakade M, Alagarasu K, Patil J,Salunke A, et al. 2015. Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses. Arch Virol 160:323-327. DOI 10.1007/s00705-014-2217-x

De la torre, J. 2015. Habrá Chikungunya para rato. Revista Semana. Disponible en, 2014. Lineamientos de vigilancia en salud pública, entomológica y de laboratorio ante la introducción del virus Chikungunya. Anexo 3 Lineamientos de vigilancia

CHKV 2014:1-15. (Internet). (Último acceso 30 de Marzo 2015). Disponible en

INS, 2015. Actualización de lineamientos de la notificación de casos de virus
Chikungunya en Colombia Fase II. Circular externa 0001.(Internet) (Último acceso 27 de abril 2015) Disponible en

INS. 2014. Vigilancia rutinaria por eventos municipal. (Internet). (Último acceso 30 de
Marzo 2015). Disponible en

INS. 2015. Vigilancia rutinaria por eventos municipal.(Internet). (Último acceso 30 de Marzo 2015). Disponible en:

INS, 2016. Semana epidemiológica número 52 de 2015. Boletín Epidemiológico semanal. 27 dic. Al 02 enero 2016: 26-30.(Internet). Disponible en:

Kashyap R, Morey S, Chandak N, Purohit H, Taori G, et al. 2010 Detection of viral antigen, IgM and IgG antibodies in cerebrospinal fluid of Chikungunya patients with neurological complications. Cerebrospinal Fluid Research. (Último acceso 13 mayo 2015). DOI 10.1186/1743-8454-7-12

Lo Presti A, Lai A, Cella E, Zehender G, Ciccozzi M. 2014. Chikungunya virus, epidemiology, clinics and phylogenesis: A review. Asian Pac J Trop Med 7(12): 925-932. DOI 10.1016/S1995-7645(14)60164-4

Loreto Horcada M, Diaz caldero C, Garrido L. 2014. Fiebre chikungunya. Manifestaciones reumáticas de una infección emergente en Europa. Reumatol Clin 11(3): 161-164. Disponible en:

Ministerio de Salud y Protección Social, Instituto Nacional de Salud. 2014.Plan deRespuesta a la Fiebre por el Virus Chikungunya. Disponible en:

Mishra B, Sharma M, Pujhari S, Ratho R, Gopal D, et al. 2011. Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and Chikungunya viruses in clinical samples. Diagn Microbiol Infect Dis 71:118–125. DOI 10.1016/j.diagmicrobio.2011.06.020

Nkoghe D, Kassa R, Bisvigou U, Caron M, Grard G. 2012. No clinical or biological difference between Chikungunya and Dengue Fever during the 2010 Gabonese outbreak. Infect Dis Rep 4(5):11-13. DOI 10.4081/idr.2012.e5

Okabayashi T, Sasaki T,Masrinoul P, Chantawat N, Yoksan S, et al. 2015. Detection of Chikungunya Virus Antigen by a Novel RapidImmunochromatographic. J Clin Microbiol 53(2): 382-388. DOI 10.1128/JCM.02033-14

Organización Panamericana de la Salud. 2012. Nueva guía de preparación y respuesta ante eventual introducción del virus chicungunya.

Organización Panamericana de la Salud. 2017. Numero de los casos reportados de fiebre chikungunya en las Americas- SE 51. (Internet) (Último acceso 24 de Enero 2018) Disponible en

Pongsiri P, Praianantathavorn K, Theamboonlers A, Payungporn S, Poovorawan Y. 2012. Multiplex real –time RT-PCR for detecting Chikungunya virus and Dengue virus. Asian Pac J Trop Med 5(5): 342-346. DOI 10.1016/S1995-7645(12)60055-8

Prat CM, Flusin O, Panella A, Tenebray B, Lanciotti R, Leparc-Goffart I. 2014. Evaluation of Commercially Available Serologic Diagnostic Tests for Chikungunya Virus. Emerg Infect Dis 20(12): 2129-2132. DOI 10.3201/eid2012.141269

Razmy A. 2014. Clinical features of chikungunya infection in Sri Lanka. Asian Pac J Trop Dis 4(2):131-134. DOI 10.1016/S2222-1808(14)60329-7

Reddy V, Ravi V, Desai A, Parida M, Powers A, et al. 2012. Utility of IgM ELISA, TaqMan Real-Time PCR, Reverse Transcription PCR, and RT-LAMP Assay for the Diagnosis of Chikungunya Fever. J.Med. Virol. 84: 1771-1778. DOI 10.1002/jmv.23406.

Rodriguez J, Lastre G, Camargo J, Fuentes G, Bermejo J, Nieto V. Adherencia de los pacientes a la Guía de Práctica Clínica del Dengue (GPC_Dengue) en una Clínica de Barranquilla (Atl, Col). Cienc. innov. salud. 2015; 3(2):23–30. DOI 10.17081/innosa.3.2.92

Saha K, Firdaus R, Chakrabarti S, Chandra Sadhukhan P. 2013. Development of rapid, sensitive one-tube duplex RT-PCR assay for specific and differential diagnosis of Chikungunya and dengue. J Virol Methods 193:521-524. DOI 10.1128/JCM.02033-14

Yathi K, Bhasker S, Chinnamma M. 2013. Determination of B cell epitopes and evaluation of antigen capture ELISA for the earlier diagnosis of CHIK virus using antirCHIK E1 rabbit antibodies. J Immunol Methods 393: 45-52. DOI 10.1016/j.jim.2013.04.005