Usefulness of immunological and molecular methods for the diagnosis of Chikungunya virus
Main Article Content
Keywords
Chikungunya diagnóstico, RT-PCR, MAC-ELISA
Abstract
Background: Chikungunya is a virus that has caused many infectious outbreaks worldwide becoming a public health problem. It shares several similarities with the Dengue virus, are transmitted by the same vector that is a mosquito of the genus Aedes, are found in tropical and subtropical areas and present the same clinical symptoms in the acute phase of the disease, which makes its clinical diagnosis difficult. Methods: Bibliographic revision of the diagnostic methods of the virus from the world literature in searchers of database and indexed journals. Discussion: Among the results it can be said that of all the immunological techniques, the MAC ELISA formats for antibody detection and the ELISAs for antigen detection show reliable results for the identification of the virus, with respect to the molecular tests it can be affirmed that they are highly sensitive and specific techniques, with multiple advantages over immunological techniques since they can detect and quantify both viruses simultaneously. Conclusion: The best techniques are molecular tests, but it is very expensive for most laboratories, therefore, the use of ELISA assays based on antigen detection is recommended because they are specific, sensitive and can detect the virus in the acute phase of the disease.
References
Cecilia D, Kakade M, Alagarasu K, Patil J,Salunke A, et al. 2015. Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses. Arch Virol 160:323-327. DOI 10.1007/s00705-014-2217-x
De la torre, J. 2015. Habrá Chikungunya para rato. Revista Semana. Disponible en http://www.semana.com/nacion/articulo/chikungunyaINS, 2014. Lineamientos de vigilancia en salud pública, entomológica y de laboratorio ante la introducción del virus Chikungunya. Anexo 3 Lineamientos de vigilancia
CHKV 2014:1-15. (Internet). (Último acceso 30 de Marzo 2015). Disponible en http://www.minsalud.gov.co/sites/rid/Lists/Bibliot
INS, 2015. Actualización de lineamientos de la notificación de casos de virus
Chikungunya en Colombia Fase II. Circular externa 0001.(Internet) (Último acceso 27 de abril 2015) Disponible en
http://www.ins.gov.co/Noticias/Chikungunya/Circula
INS. 2014. Vigilancia rutinaria por eventos municipal. (Internet). (Último acceso 30 de
Marzo 2015). Disponible en http://www.ins.gov.co/lineasdeaccion/SubdireccionV
INS. 2015. Vigilancia rutinaria por eventos municipal.(Internet). (Último acceso 30 de Marzo 2015). Disponible en:
http://www.ins.gov.co/lineas-de-accion/Subdireccio
INS, 2016. Semana epidemiológica número 52 de 2015. Boletín Epidemiológico semanal. 27 dic. Al 02 enero 2016: 26-30.(Internet). Disponible en: http://www.ins.gov.co/boletin-epidemiologico/Bolet
Kashyap R, Morey S, Chandak N, Purohit H, Taori G, et al. 2010 Detection of viral antigen, IgM and IgG antibodies in cerebrospinal fluid of Chikungunya patients with neurological complications. Cerebrospinal Fluid Research. (Último acceso 13 mayo 2015). DOI 10.1186/1743-8454-7-12
Lo Presti A, Lai A, Cella E, Zehender G, Ciccozzi M. 2014. Chikungunya virus, epidemiology, clinics and phylogenesis: A review. Asian Pac J Trop Med 7(12): 925-932. DOI 10.1016/S1995-7645(14)60164-4
Loreto Horcada M, Diaz caldero C, Garrido L. 2014. Fiebre chikungunya. Manifestaciones reumáticas de una infección emergente en Europa. Reumatol Clin 11(3): 161-164. Disponible en: http://www.reumatologiaclinica.org/es/fiebre-chiku
Ministerio de Salud y Protección Social, Instituto Nacional de Salud. 2014.Plan deRespuesta a la Fiebre por el Virus Chikungunya. Disponible en: https://www.minsalud.gov.co/sites/rid/Lists/Biblio
Mishra B, Sharma M, Pujhari S, Ratho R, Gopal D, et al. 2011. Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and Chikungunya viruses in clinical samples. Diagn Microbiol Infect Dis 71:118–125. DOI 10.1016/j.diagmicrobio.2011.06.020
Nkoghe D, Kassa R, Bisvigou U, Caron M, Grard G. 2012. No clinical or biological difference between Chikungunya and Dengue Fever during the 2010 Gabonese outbreak. Infect Dis Rep 4(5):11-13. DOI 10.4081/idr.2012.e5
Okabayashi T, Sasaki T,Masrinoul P, Chantawat N, Yoksan S, et al. 2015. Detection of Chikungunya Virus Antigen by a Novel RapidImmunochromatographic. J Clin Microbiol 53(2): 382-388. DOI 10.1128/JCM.02033-14
Organización Panamericana de la Salud. 2012. Nueva guía de preparación y respuesta ante eventual introducción del virus chicungunya. http://www.paho.org/uru/index.php?option=com_conte
Organización Panamericana de la Salud. 2017. Numero de los casos reportados de fiebre chikungunya en las Americas- SE 51. (Internet) (Último acceso 24 de Enero 2018) Disponible en http://www.paho.org/hq/index.php?option=com_docman
Pongsiri P, Praianantathavorn K, Theamboonlers A, Payungporn S, Poovorawan Y. 2012. Multiplex real –time RT-PCR for detecting Chikungunya virus and Dengue virus. Asian Pac J Trop Med 5(5): 342-346. DOI 10.1016/S1995-7645(12)60055-8
Prat CM, Flusin O, Panella A, Tenebray B, Lanciotti R, Leparc-Goffart I. 2014. Evaluation of Commercially Available Serologic Diagnostic Tests for Chikungunya Virus. Emerg Infect Dis 20(12): 2129-2132. DOI 10.3201/eid2012.141269
Razmy A. 2014. Clinical features of chikungunya infection in Sri Lanka. Asian Pac J Trop Dis 4(2):131-134. DOI 10.1016/S2222-1808(14)60329-7
Reddy V, Ravi V, Desai A, Parida M, Powers A, et al. 2012. Utility of IgM ELISA, TaqMan Real-Time PCR, Reverse Transcription PCR, and RT-LAMP Assay for the Diagnosis of Chikungunya Fever. J.Med. Virol. 84: 1771-1778. DOI 10.1002/jmv.23406.
Rodriguez J, Lastre G, Camargo J, Fuentes G, Bermejo J, Nieto V. Adherencia de los pacientes a la Guía de Práctica Clínica del Dengue (GPC_Dengue) en una Clínica de Barranquilla (Atl, Col). Cienc. innov. salud. 2015; 3(2):23–30. DOI 10.17081/innosa.3.2.92
Saha K, Firdaus R, Chakrabarti S, Chandra Sadhukhan P. 2013. Development of rapid, sensitive one-tube duplex RT-PCR assay for specific and differential diagnosis of Chikungunya and dengue. J Virol Methods 193:521-524. DOI 10.1128/JCM.02033-14
Yathi K, Bhasker S, Chinnamma M. 2013. Determination of B cell epitopes and evaluation of antigen capture ELISA for the earlier diagnosis of CHIK virus using antirCHIK E1 rabbit antibodies. J Immunol Methods 393: 45-52. DOI 10.1016/j.jim.2013.04.005
